phosphorylated stat3 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc phosphorylated stat3

( a ) Isolation and characterization of UMSC-EVs. ( b ) Isolation and characterization of EMSC-EVs. ( c ) Characterization of UMSC-EVs and EMSC-EVs by WB. ( d ) AA mouse model. ( e ) H&E staining of skin from AA mice. ( f ) H&E staining of skin from healthy mice. ( g ) Hair growth in each group on day 15. ( h ) Tracing results of EMSC-EVs (green fluorescence). ( i ) Venn diagram. ( j ) Volcano plot. k . Bubble plot of pathway enrichment analysis. l . miR-665 expression in UMSC-EVs and EMSC-EVs. m . Preliminary screening of miRNAs. n . Target prediction of miR-665 via miRDB database. o . Mechanism of miR-665 targeting <t>STAT3</t> mRNA. p . The results of the dual-luciferase reporter assay. ( n = 3 per group; ns = not significant, *** P < 0.001, **** P < 0.0001)

Phosphorylated Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+smad2/PathScan+Total+Smad2%2F3+Sandwich+ELISA+Kit/pmc13032648-151-9-15
Average 96 stars, based on 180 article reviews

phosphorylated stat3 - by Bioz Stars, 2026-09

96/100 stars

Images

1) Product Images from "ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata"

Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-026-04214-7

Figure Legend Snippet: ( a ) Isolation and characterization of UMSC-EVs. ( b ) Isolation and characterization of EMSC-EVs. ( c ) Characterization of UMSC-EVs and EMSC-EVs by WB. ( d ) AA mouse model. ( e ) H&E staining of skin from AA mice. ( f ) H&E staining of skin from healthy mice. ( g ) Hair growth in each group on day 15. ( h ) Tracing results of EMSC-EVs (green fluorescence). ( i ) Venn diagram. ( j ) Volcano plot. k . Bubble plot of pathway enrichment analysis. l . miR-665 expression in UMSC-EVs and EMSC-EVs. m . Preliminary screening of miRNAs. n . Target prediction of miR-665 via miRDB database. o . Mechanism of miR-665 targeting STAT3 mRNA. p . The results of the dual-luciferase reporter assay. ( n = 3 per group; ns = not significant, *** P < 0.001, **** P < 0.0001)

Techniques Used: Isolation, Staining, Fluorescence, Expressing, Luciferase, Reporter Assay

( a ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs. ( b ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs before and after IFN-γ treatment. ( c ) STAT3 expression in IFN-γ-treated HaCaT cells and DPCs. ( d ) WB results showing STAT3 expression in lentivirus-transfected HaCaT cells before and after IFN-γ treatment. ( e ) WB results showing STAT3 expression in lentivirus-transfected DPCs before and after IFN-γ treatment. ( f ) Scratch assay results of HaCaT cells. ( g ) 48 h Transwell assay results of DPCs. ( h ) WB results of rescue experiments in HaCaT cells and DPCs. ( i ) Growth of hair follicles in ex vivo culture on day 5 under different treatment conditions. ( n = 3–6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001)

Figure Legend Snippet: ( a ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs. ( b ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs before and after IFN-γ treatment. ( c ) STAT3 expression in IFN-γ-treated HaCaT cells and DPCs. ( d ) WB results showing STAT3 expression in lentivirus-transfected HaCaT cells before and after IFN-γ treatment. ( e ) WB results showing STAT3 expression in lentivirus-transfected DPCs before and after IFN-γ treatment. ( f ) Scratch assay results of HaCaT cells. ( g ) 48 h Transwell assay results of DPCs. ( h ) WB results of rescue experiments in HaCaT cells and DPCs. ( i ) Growth of hair follicles in ex vivo culture on day 5 under different treatment conditions. ( n = 3–6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001)

Techniques Used: Expressing, Transfection, Wound Healing Assay, Transwell Assay, Ex Vivo

Control:

Article Title: IFN-γ and TNF-α Impair Lung Development by Upregulating SMAD7 to Inhibit TGF-β Signaling Pathway and ECM Dysregulation
Article Snippet: .. Primary antibodies were used as follows: α-smooth muscle actin (α-SMA, Cat#19245, 1:1000), collagen type I alpha 1 chain (COL1A1, Cat#91144, 1:1000), SMAD2 (Cat#5339, 1:1000), phosphorylated SMAD2 (p-SMAD2, Ser465/467, Cat#18338, 1:1000), SMAD3 (Cat#9523, 1:1000), phosphorylated SMAD3 (p-SMAD3, Ser423/425, Cat#9520, 1:1000), and SMAD4 (Cat#38454, 1:1000) from Cell Signaling Technology (CST, USA); SMAD7 (Cat#ab216428, 1:1000) from Abcam (UK); β-actin (1:1000) as loading control from Beijing Boaosen Biotechnology (Beigjing, China). .. Total RNA was extracted from cells/tissues with TRIzol reagent (Thermo Fisher Scientific) following the supplier’s instructions.

Membrane:

Article Title: Tacrolimus Inhibits Human Tenon’s Fibroblast Migration, Proliferation, and Transdifferentiation
Article Snippet: .. Monoclonal mouse antibodies against α-SMA (1:1000; A2228, Sigma-Aldrich, Inc.), PCNA (1:1000; 2586, Cell Signaling Technology, Inc., Beverly, MA, USA), Smad2 (1:1000; 3103, Cell Signaling Technology, Inc.), phosphorylated Smad2 (pSmad2)(1:1000; 3104, Cell Signaling Technology, Inc.), Smad3 (1:1000; sc-101154, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), phosphorylated Smad3 (pSmad3)(1:1000; sc-517575, Santa Cruz Biotechnology, Inc.), ERK (1:1000; sc-271269, Santa Cruz Biotechnology, Inc.), phosphorylated ERK (pERK)(1:1000; sc-81492, Santa Cruz Biotechnology, Inc.), Akt (1:1000; sc-5298, Santa Cruz Biotechnology, Inc.), phosphorylated Akt (pAkt)( 1:1000; sc-377556, Santa Cruz Biotechnology, Inc.), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)(1:1000; 5174, Cell Signaling Technology, Inc.) were added to the previously transferred membrane and allowed to stand at 4 ◦C overnight. .. The next day, after washing the membrane with blocking solution, horse radish peroxidaseconjugated secondary antibodies (1:5000; 31430, Invitrogen-Gibco-Life Science Technology, Inc.) were added and reacted at room temperature for 1 h. After 1 h, after washing the membrane thrice with a blocking solution, it was transferred to an enhanced chemiluminescence detection system (sc-2048, Santa Cruz Biotechnology, Inc.).

Article Title: Tacrolimus Inhibits Human Tenon’s Fibroblast Migration, Proliferation, and Transdifferentiation
Article Snippet: .. Monoclonal mouse antibodies against α-SMA (1:1000; A2228, Sigma-Aldrich, Inc.), PCNA (1:1000; 2586, Cell Signaling Technology, Inc., Beverly, MA, USA), Smad2 (1:1000; 3103, Cell Signaling Technology, Inc.), phosphorylated Smad2 (pSmad2)(1:1000; 3104, Cell Signaling Technology, Inc.), Smad3 (1:1000; sc-101154, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), phosphorylated Smad3 (pSmad3)(1:1000; sc-517575, Santa Cruz Biotechnology, Inc.), ERK (1:1000; sc-271269, Santa Cruz Biotechnology, Inc.), phosphorylated ERK (pERK)(1:1000; sc-81492, Santa Cruz Biotechnology, Inc.), Akt (1:1000; sc-5298, Santa Cruz Biotechnology, Inc.), phosphorylated Akt (pAkt)( 1:1000; sc-377556, Santa Cruz Biotechnology, Inc.), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)(1:1000; 5174, Cell Signaling Technology, Inc.) were added to the previously transferred membrane and allowed to stand at 4 °C overnight. .. The next day, after washing the membrane with blocking solution, horse radish peroxidase-conjugated secondary antibodies (1:5000; 31430, Invitrogen-Gibco-Life Science Technology, Inc.) were added and reacted at room temperature for 1 h. After 1 h, after washing the membrane thrice with a blocking solution, it was transferred to an enhanced chemiluminescence detection system (sc-2048, Santa Cruz Biotechnology, Inc.).

Immunodetection:

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling
Article Snippet: SDS-page gels (BioRad; 4–20%) were loaded with 14.75 μg of protein/well and semi-dry transferred to 0.2 μm PVDF membrane (BioRad), using standard procedures . .. Immunodetection included phosphorylated SMAD2 (CellSignalling C#3108S, 1:1000) as a marker of TGF β -1 signalling and GAPDH (CellSignalling C#2118S, 1:1000) as a reference. .. A secondary horseradish peroxide conjugated goat anti-rabbit antibody (CellSignalling C#7074S, 1:2000) and ECL Substrate-Luminol Solution (Ultrasense, Froggobio) were used to detect the primary antibody.

Article Title: The regulation of placental pericyte function through transforming growth factor β-1 signalling.
Article Snippet: SDS-page gels (BioRad; 4–20%) were loaded with 14.75 μg of protein/well and semidry transferred to 0.2 μm PVDF membrane (BioRad), using standard procedures61. .. Immunodetection included phosphorylated SMAD2 (CellSignalling C#3108S, 1:1000) as a marker of TGFβ-1 signalling and GAPDH (CellSignalling C#2118S, 1:1000) as a reference. .. A secondary horseradish peroxide conjugated goat anti-rabbit antibody (CellSignalling C#7074S, 1:2000) and ECL Substrate-Luminol Solution (Ultrasense, Froggobio) were used to detect the primary antibody.

Marker:

Incubation:

Article Title: Self-homing TGF-β1 traps: Engineered vasorin vesicles as smart biomaterials for targeted anti-fibrotic therapy after myocardial infarction
Article Snippet: .. Membranes were then incubated overnight at 4 °C with the following primary antibodies: anti-Flag (1:5,000; ABclonal, Hangzhou, China), β3-tubulin (1:10,000; Cell Signaling Technology, Danvers, MA, USA), integrin β3/CD61 (1:1,000; Cell Signaling Technology), matrix metalloproteinase-2 (MMP-2) (1:1,000; Cell Signaling Technology), fibronectin (1:1,000; ABclonal), collagen I (COL1) (1:1,000; ABclonal), α-smooth muscle actin (α-SMA) (1:1,000; Cell Signaling Technology), drosophila mothers against decapentaplegic 2 (SMAD2) (1:1,000; Cell Signaling Technology), phosphorylated SMAD2 (1:1,000; Cell Signaling Technology), and vasorin (1:1,000; Thermo Fisher Scientific Inc.). .. After three 5-min TBST washes, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse or anti-rabbit, 1:5,000; Thermo Fisher Scientific Inc.) for 1 h at RT.

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